. Untersuchungen im Feld von Bacillus thuringiensis zur Steuerung von Western Hemlock looper. Hemlock looper Kontrolle; Bacillus thuringiensis. Verhältnis von SPRAY KAUTION UND DIE VERTEILUNG DER STERBLICHKEIT Methoden am Tag der Spritzen, wurden Proben von je 5 Gallone Trommel die lebensfähigen Sporen Konzentration des unverdünnten Thuricide zu bestimmen. Diese wurden durch Verdünnen der Sporensuspension verarbeitet, Kultivierung der Sporen und die Zählung der Bazillus, der Kolonien. Abbildung 6.-Sperrholz Fall verwendet spray Einlagen Schützen und Transportieren von Glasplatten in das Feld ein. Bei jeder Handlung in den behandelten Bereich, acht 5 x 7-Zoll-Glas

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. Field testing of Bacillus thuringiensis for control of western hemlock looper. Hemlock looper Control; Bacillus thuringiensis. RELATION OF SPRAY DEPOSIT AND DISTRIBUTION TO MORTALITY Methods On the day of spraying, samples were taken from each 5-gallon drum to determine the viable spore concentration of the undiluted Thuricide. These were processed by diluting the spore suspension, culturing the spores, and counting the Bacillus colonies.. Figure 6.—Plywood case used to protect spray deposits while transporting glass plates in the field. At each plot in the treated area, eight 5x7-inch glass plates were used to collect spray deposit, and eight 5x7-inch cards of a selected cover stock were placed to record spray distribution. To insure exposure of slides and cards to falling spray, two small clearings were made at each plot under existing openings in the forest canopy. Four plates and four cards were placed in an opening up- hill and four of each in an opening down- hill from the access line at each plot, on surfaces above the ground such as logs and stumps. After the spraying, plates were transported in specially made ply- wood cases (fig. 6) to Oregon State Uni- versity, where number of viable spores was determined.^' Cards were brought immediately to our Portland headquarters and photographed under ultraviolet light to obtain a permanent record of numbers and sizes of spray particles. An indica- tion of spray distribution in the lower crown of sample trees was obtained by examining foliage samples in a darkened room with a portable ultraviolet lamp. The persistence of Bacillus in field deposits was analyzed at a field laboratory by two methods. In one, leaves of salal (Gaultheria shallon Pursh) were collected daily for 20 days following spraying. One-inch squares were cut from these leaves and washed with 10 milliliters of distilled water. After the washing was diluted 1:10, 1:100, and 1:1000, 0.1 milliliter of each dilution was — Contract arrangement with M

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